Apoptosis

Description: B-HT 920, marketed as talipexole in Japan, is a full agonist at both pre- and postsynaptic D-2 dopamine receptors agonist and ?2-adrenergic receptor agonist. In addition, it is a 5-HT3 receptor antagonist in both rat cortical and intestinal membrane fractions.

Description: B-HT 920, marketed as talipexole in Japan, is a full agonist at both pre- and postsynaptic D-2 dopamine receptors agonist and ?2-adrenergic receptor agonist. In addition, it is a 5-HT3 receptor antagonist in both rat cortical and intestinal membrane fractions.

Description: B-HT 920, marketed as talipexole in Japan, is a full agonist at both pre- and postsynaptic D-2 dopamine receptors agonist and ?2-adrenergic receptor agonist. In addition, it is a 5-HT3 receptor antagonist in both rat cortical and intestinal membrane fractions.

Description: HT-29 lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The HT-29 lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.